Journal: bioRxiv

Article Title: Hyperglycemic state and fluid shear stress affect metastatic breast cancer cell migration via focal adhesion kinase

doi: 10.1101/2025.05.22.655615

Figure Lengend Snippet: Co-centered cell migration tracks (a) and corresponding vector plots (b) for fluid flowed cells under high glucose without or with FAK inhibitor (FAK14). Fluid shear-induced MDA-MB-231 cell migration length under high glucose was significantly suppressed by FAK14 (c) (FF15: 15 dyne/cm 2 ). The migration inhibitory effects of FAK14 were also seen in confinement ratio (d) and arrest coefficient (e). Static MCF-10A cell data under low glucose were shown as controls for comparison. *, ***: p < 0.05 and 0.001, respectively. Bar graphs are plotted as mean ± SEM.

Article Snippet: For FAK inhibition studies, slides were placed in media containing 10 μM FAK inhibitor 14 (FAK14, Santa Cruz, sc-203950, Dallas, TX, USA) for 1 h. Then, slides were rinsed in phosphate buffered saline (PBS) before being placed in the flow chamber.

Techniques: Migration, Plasmid Preparation, Shear, Comparison

Journal: bioRxiv

Article Title: Hyperglycemic state and fluid shear stress affect metastatic breast cancer cell migration via focal adhesion kinase

doi: 10.1101/2025.05.22.655615

Figure Lengend Snippet: FAK inhibition by FAK14 significantly decreased MDA-MB-231 cell migration speed under fluid shear (FF15: 15 dyne/cm 2 ) and high glucose (a); t is the time in min after the fluid shear onset. Overall migration of the group was also blocked by FAK14 as in the RMS vs. t 1/2 plot (b). Motility coefficient was Static: 0.84, FF15: 1.76, and FF15-FAK14: 0.86, all under high glucose. ***: p < 0.001, Bar graphs are plotted as mean ± SEM.

Article Snippet: For FAK inhibition studies, slides were placed in media containing 10 μM FAK inhibitor 14 (FAK14, Santa Cruz, sc-203950, Dallas, TX, USA) for 1 h. Then, slides were rinsed in phosphate buffered saline (PBS) before being placed in the flow chamber.

Techniques: Inhibition, Migration, Shear

Journal: bioRxiv

Article Title: Hyperglycemic state and fluid shear stress affect metastatic breast cancer cell migration via focal adhesion kinase

doi: 10.1101/2025.05.22.655615

Figure Lengend Snippet: (a) Initial scratch area at 0 h and the area imaged at 24 h under low (L) and high (H) glucose without or with FAK inhibitor (FAK14). There was no appreciable difference in scratch wound area with low (L) and high (H) glucose conditions. On the other hand, FAK14 considerably suppressed the covering of the scratch (b) and movement of cells towards the scratch (c). **: p < 0.01 (n = 5). Bar graphs are plotted as mean ± SEM.

Article Snippet: For FAK inhibition studies, slides were placed in media containing 10 μM FAK inhibitor 14 (FAK14, Santa Cruz, sc-203950, Dallas, TX, USA) for 1 h. Then, slides were rinsed in phosphate buffered saline (PBS) before being placed in the flow chamber.

Techniques:

Journal: bioRxiv

Article Title: Hyperglycemic state and fluid shear stress affect metastatic breast cancer cell migration via focal adhesion kinase

doi: 10.1101/2025.05.22.655615

Figure Lengend Snippet: (a) Images of transwell migration assay for MDA-MB-231 cells under low (L) and high (H) glucose without or with FAK inhibitor (FAK14). Arrows example the regions of stained cells. (b) The invasive cell count was not affected by glucose concentration, while FAK14 substantially decreased cell movement across the membrane. Invasive cell count is presented as % of low glucose control without FAK14. *: p < 0.05 (n = 5). Bar graphs are plotted as mean ± SEM.

Article Snippet: For FAK inhibition studies, slides were placed in media containing 10 μM FAK inhibitor 14 (FAK14, Santa Cruz, sc-203950, Dallas, TX, USA) for 1 h. Then, slides were rinsed in phosphate buffered saline (PBS) before being placed in the flow chamber.

Techniques: Transwell Migration Assay, Staining, Cell Counting, Concentration Assay, Membrane, Control